Association of 2'-5' oligoribonucleotides.
نویسندگان
چکیده
Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imino proton NMR spectra and NOEs for (2'-5') CGGCGCCG are consistent with formation of an antiparallel duplex. The results suggest greater duplex stability was one factor favoring 3'-5' over 2'-5' linkages in evolution.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 20 7 شماره
صفحات -
تاریخ انتشار 1992